MERCK 110493 BHI (Brain Heart Infusion) broth acc. ISO 6888 GranuCult 500 gr
Catalogue Number	110493
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ100

Physicochemical Information
pH value	7.2 - 7.6 (37 g/l, H₂O, 37 °C) (after autoclaving)
Bulk density	730 kg/m3
Solubility	37 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15 °C to +25 °C.

Specifications
Appearance (colour)	brown
Appearance (clearness)	clear
pH-value (25 °C)	7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034))	≤ 100
Inoculum on reference medium (Streptococcus pneumoniae ATCC 6305)	≤ 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 19615)	≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	≤ 100
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054))	≤ 100
Inoculum on reference medium (Bacteroides fragilis ATCC 25285)	≤ 100
Inoculum on reference medium (Haemophilus influenzae ATCC 33391)	≤ 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034))	good to very good
Growth (Streptococcus pneumoniae ATCC 6305)	good to very good
Growth (Streptococcus pyogenes ATCC 19615)	good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	good to very good
Growth (Candida albicans ATCC 10231 (WDCM 00054))	good to very good
Growth (Bacteroides fragilis ATCC 25285)	good to very good
Growth (Haemophilus influenzae ATCC 33391)	good to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C aerobic C.albicans 44 ± 4 hours at 37 ± 1 °C aerobic B.fragilis 44 ± 4 hours at 37 ± 1 °C anaerobic H.influenza 44 ± 4 hours at 37 ± 1 °C microaerophilic and with addition of X+V-factor.

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