Fraser Listeria Ammonium İron(III) Supplement Merck 100092 10 X 1 Vial

Fraser Listeria Ammonium İron(III) Supplement Merck 100092 10 X 1 Vial
Catalogue Number	100092
Replaces	1000920017

Product Information
CAS number	1185-57-5
EC number	214-686-6
Hill Formula	C₆H₈O₇*xFe*xH₃N
HS Code	2918 15 00
Quality Level	MQ100

Applications
Application	FRASER Listeria Ammonium iron(III) Supplement. CAS 1185-57-5, pH 6 - 8 (100 g/l, H₂O, 20 °C).

Physicochemical Information
pH value	6 - 8 (100 g/l, H₂O, 20 °C)
Solubility	1200 g/l

Toxicological Information
LD 50 oral	LD50 Rat > 2000 mg/kg

Safety Information according to GHS
RTECS	GE7540000
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15 °C to +25 °C.

Transport Information
Declaration (railroad and road) ADR, RID	Kein Gefahrgut
Declaration (transport by air) IATA-DGR	No Dangerous Good
Declaration (transport by sea) IMDG-Code	No Dangerous Good

Specifications
Growth promotion test according to ISO 11133 Mixed inoculum of Listeria, E.coli and E.faecalis Additionaly monoculture of E.coli and E.faecalis Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium - Listeria monocytogenes ATCC 19111 (WDCM 00020)	10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013)	≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012)	≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	≥ 1000
Blacking - Listeria monocytogenes ATCC 19111 (WDCM 00020)	+
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	+
- Escherichia coli ATCC 25922 (WDCM 00013)	-
- Escherichia coli ATCC 8739 (WDCM 00012)	-
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	-
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	-
Growth on ALOA® medium - Listeria monocytogenes ATCC 19111 (WDCM 00020)	> 10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	> 10 CFU
Colonies - Listeria monocytogenes ATCC 19111 (WDCM 00020)	bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	bluegreen with non transparent zone
Growth on Tryptic soy agar - Escherichia coli ATCC 25922 (WDCM 00013)	no growth
- Escherichia coli ATCC 8739 (WDCM 00012)	no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	< 100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	< 100 CFU
Incubation: Half Fraser Broth: 24 hours at 30°C, aerobic Fraser Broth: 48 hours at 37°C, aerobic Confirmation on ALOA®-Medium: 48 hours at 37°C, aerobic Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic