Appearance (clearness) |
clear |
Appearance (colour) |
yellowish to yellowish-brown |
Solidification behaviour (2 hrs., 40 °C) |
liquid |
Solidification behaviour (4 hrs., 45 °C) |
liquid |
pH-value (25 °C) |
6.8 - 7.2 |
Growth promotion test in accordance with the current version of DIN EN ISO 11133. |
Incubation: 72 ± 3 hours at 30 ± 1 °C; aerobic |
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
|
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) |
|
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
|
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) |
|
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) |
|
Inoculum on reference medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)) |
10 - 100 |
Inoculum on reference medium (Listeria monocytogenes ATCC 19118) |
10 - 100 |
Inoculum on reference medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)) |
10 - 100 |
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
|
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)) |
|
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)) |
|
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)) |
|
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)) |
|
Colony count (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)) |
|
Colony count (Listeria monocytogenes ATCC 19118) |
|
Colony count (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)) |
|
Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
≥ 70 % |
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) |
≥ 70 % |
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
≥ 70 % |
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)) |
≥ 70 % |
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) |
≥ 70 % |
Recovery on test medium (Lactococcus lactis spp. lactis ATCC 19435 (WDCM 00016)) |
≥ 70 % |
Recovery on test medium (Listeria monocytogenes ATCC 19118) |
≥ 70 % |
Recovery on test medium (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)) |
≥ 70 % |
Incubation: 24 hours at 35 - 37 °C; aerobic |
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
|
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) |
|
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
|
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) |
|
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) |
|
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
|
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)) |
|
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)) |
|
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)) |
|
Colony count (Bacillus subtilis ATCC 6633 (WDCM 00003)) |
|
Recovery (Staphylococcus aureus ATCC 6538 (WDCM 00032)) |
≥ 70 % |
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) |
≥ 70 % |
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)) |
≥ 70 % |
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)) |
≥ 70 % |
Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)) |
≥ 70 % |
Reference: Tryptic soy agar.
S.aureus, E.coli and B.subtilis tested with pour plate technic.
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Other strains in single determination with spiral platemethode. |