Description
Catalogue Number	109261
Synonyms	Iodine-potassium iodide solution
Description	LUGOL'S SOLUTION (DILUTED IODINE-POTASS
Overview	In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with Gram's iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A 500 mL PE-bottle of Gram's Lugol's solution is sufficient for staining up to 250 slides. Lugol’s solution is also a part of the Gram-color stain set. It is an IVD & CE product.

Product Information
HS Code	3822 00 00

Physicochemical Information
Boiling point	100 °C (1013 hPa)
Density	1.01 g/cm3 (20 °C)
pH value	3.5 (H₂O, 20 °C)

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	NWG not water endangering

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Suitability for microscopy	passes test
Staphylococcus aureus	dark bluish violet
E.coli	pink to red

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MERCK 109261 Lugol's solution (diluted iodine-potassium iodide solution) for the Gram staining method

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