MERCK 115943 Methylene blue (C.I.52015) for microscopy Certistain

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Stok Kodu
115943.0025
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Description
Catalogue Number 115943
Synonyms Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
Description METHYLENE BLUE (C.I. 52015) FOR MICROSCO
Overview Methylene blue (C.I. 52015) is a Certistain® dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration.
Product Information
EC number 200-515-2
Hill Formula C₁₆H₁₈ClN₃S * x H₂O
Chemical formula C₁₆H₁₈ClN₃S * x H₂O (x=2-3)
Molar Mass 319.86 g/mol (anhydrous)
HS Code 3204 13 00
Structure formula Image
Physicochemical Information
Melting Point 180 °C (decomposition)
pH value 3 (10 g/l, H₂O, 20 °C)
Bulk density 400 - 600 kg/m3
Solubility 50 g/l
Toxicological Information
LD 50 oral LD50 Rat 1180 mg/kg
Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s) H302: Harmful if swallowed.
Signal Word Warning
Storage class 10 - 13 Other liquids and solids
WGK WGK 2 water endangering
Disposal 3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Hazard Symbols Harmful
Categories of danger harmful
R Phrase R 22
Harmful if swallowed.
Storage and Shipping Information
Storage Store at +5°C to +30°C.
Specifications
Dye content (spectrophotometrically) ≥ 82 %
Identity
- Chloride

passes test
- UV/VIS-Spectrum passes test
Absorption maximum λ max (ethanol 50 %) 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %) 2250 - 2750
TLC-Test passes test
Loss on drying (110 °C) 10 - 15 %
Suitability for microscopy passes test
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MERCK 115943 Methylene blue (C.I.52015) for microscopy Certistain

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