Sigma Aldrich T1503 Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline Cas 77-86-1 1 kg
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T1503-1KG
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Sigma Aldrich T1503 Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline Cas 77-86-1 1 kg | |||||||
Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
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Linear Formula: NH2C(CH2OH)3
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CAS Number:
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77-86-1 | ||||||
General description
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40% (w/w) solution in water is clear and colorless.
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Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
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Application
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Trizma® base has been used:
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as a component of H buffer (cell dissociation buffer) | |||||||
for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique | |||||||
as an assay buffer for reconstitution of extracted and dried protein samples | |||||||
to prepare Tris-HCl buffer that is used to stabilize proteins | |||||||
as a buffer to extract carotenoid from tubers | |||||||
as a component of sample buffer during protein extraction prior to western blotting | |||||||
as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) | |||||||
to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay | |||||||
as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate
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