Tsc (Tryptose Sulfite Cycloserine) Agar (Base) Acc. Iso 7937 And Iso 14189 granucult™ Merck 111972 500 gr

Tsc (Tryptose Sulfite Cycloserine) Agar (Base) Acc. Iso 7937 And Iso 14189 granucult™ Merck 111972  500 gr
Catalogue Number	111972
Synonyms	TSC-agar, Tryptose sulfite cycloserine agar
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ100

Physicochemical Information
pH value	7.4 - 7.8 (H₂O, 25 °C)
Bulk density	660 kg/m3
Solubility	42 g/l

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H319: Causes serious eye irritation.
Precautionary Statement(s)	P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal Word	Warning
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15 °C to +25 °C.

Specifications
Appearance (clearness)	clear
Appearance (color)	brown
Solidification behaviour (2 hrs., 45 °C)	liquid
pH-value (25 °C)	7.4 - 7.8
Typical composition (g/litre): Tryptose 15.0; Peptone from soymeal 5.0; Yeast extract 5.0; Sodium dusilfit 1.0; Ammonium iron(III) citrate 1.0; Agar-agar 12.0.
Growth promotion test in accordance with ISO 11133. Base tested with supplement Art. 1.00888.
Inoculum on reference medium (Clostridium perfringens ATCC 10543)	10 - 100
Inoculum on reference medium (Clostridium perfringens ATCC 13124 (WDCM 00007))	10 - 100
Inoculum on reference medium (Clostridium perfringens ATCC 12916 (WDCM 00080))	10 - 100
Inoculum on reference medium (Clostridium tetani ATCC 19406)	> 10⁴
Inoculum on reference medium (Clostridium novyi ATCC 17861)	> 10⁴
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	> 10⁴
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001))	> 10⁴
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	> 10⁴
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	> 10⁴
Colony count (Clostridium perfringens ATCC 10543)
Colony count (Clostridium perfringens ATCC 13124 (WDCM 00007))
Colony count (Clostridium perfringens ATCC 12916 (WDCM 00080))
Recovery on test medium (Clostridium perfringens ATCC 10543)	≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007))	≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 12916 (WDCM 00080))	≥ 70 %
Growth (Clostridium tetani ATCC 19406)	none to fair
Growth (Clostridium novyi ATCC 17861)	none to fair
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	none to poor
Growth (Bacillus cereus ATCC 11778 (WDCM 00001))	none to fair
Growth (Escherichia coli ATCC 25922 (WDCM 00013))	inhibited
Growth (Escherichia coli ATCC 8739 (WDCM 00012))	inhibited
Blacking (Clostridium perfringens ATCC 10543)	+
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007))	+
Blacking (Clostridium perfringens ATCC 12916 (WDCM 00080))	+
Blacking (Clostridium tetani ATCC 19406)	-
Blacking (Clostridium novyi ATCC 17861)	-
Blacking (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	-
Blacking (Bacillus cereus ATCC 11778 (WDCM 00001))	-
Blacking (Escherichia coli ATCC 25922 (WDCM 00013))	-
Blacking (Escherichia coli ATCC 8739 (WDCM 00012))	-
Fluorescence (Clostridium perfringens ATCC 10543)	+
Fluorescence (Clostridium perfringens ATCC 13124 (WDCM 00007))	+
Fluorescence (Clostridium perfringens ATCC 12916 (WDCM 00080))	+
Incubation: 20 hrs. 37 °C. Reference: TSC-agar for Cl.perfingens; Blood-agar for all other strains.

 

 

 

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